Fermenter bioreactor manufacturers, Fermentor and Bioreactor manufacturer
Fermenter and Bioreactor manufacturer –
The solid state fermentation has broad applications in medicine, beverage industry, enzyme preparations, biological pesticide, etc. This fermenter system has the volumes ranging from 5L-20KL with advantages of low investment, good aseptic performance, safe operation environment, simple maintenance, no environmental pollution, etc.
Mixing method: magnetic stirring or mechanical stirring one of the two optional
Sterilization method: on-site sterilization auto control or manual control
Control system: Siemens PLC, industrial LCD touch computer control, parameters can be set to store, can be remotely connected to the computer through the USB interface, the data
Temperature detection and control
Heat by thermostatic water tank and circulate by circulating pump (Grundfos, Denmark), auto control (cooling water+5℃) to 65℃±0.1℃,temperature probe (PT100)
pH detection and control
2.00-12.00±0.05 pH, auto control by adding acid and base, pH sensor (Mettler/Hamilton, Switzerland) and shielding lead (Mettler/Hamilton, Switzerland). fermenter is auto controlled with peristaltic pump.
Stirring system
Mechanical sealing system: high-powered stirring oar, anti-foam oar; AC electrical machine: infinite speed variation; rpm in the fermenter: 50~1000 rpm±1%, The height of the stirring oar can be adjusted.
DO detection and control
0-150±3% displaying precision 0.1%, sensor (Mettler/Hamilton, Switzerland) and shielding lead(Mettler/Hamilton, Switzerland)
Feed control
Peristaltic pump to add materials
Anti-foam control
Tested by sensor and added anti foamer by peristaltic pump.
Intake control (air) manual control Rota meter
Lid lift: Have lid lift system.
Harvest valve: Zero dead leg flush bottom valves, can be sterilized separately, manual.
Sampling valve: At the low side of vessel, can be sterilized separately.
Exhaust: Condenser before the exhaust, exhaust controlled by diagram valve
Pressure detection and control
Auto control, pressure transmitter from BD, Germany, and displayed by pressure gauge
Pipeline and valve
The pipelines and valves which are in contact with the fluid are made by 316L stainless steel, the valves are diaphragm cell valves.
Material: 316L stainless steel inside and 304 jacket
The diameter and height ratio: 1:2-3
The large-scale culture technology of animal cells (hybridoma cells, CHO cells, insect cells, etc.) and its bioreactor engineering system can be widely used in research, process development and industrial production of antibodies, recombinant protein drugs, viral vaccines and other biotechnological products.
Working volume: 10/20/30/50/80/100/200L/500L/1000L/5000L
Main body material: 316L stainless steel
Stainless Steel Bioreactors & Fermenters Manufacturer
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The key difference between bioreactor and fermentor is the type of reaction that facilitates by each closed system. Bioreactor facilitates any type of biochemical reactions while fermentor facilitates fermentation. In fact, fermentors are specific bioreactors designed for fermentation reactions that occur under anaerobic conditions. However, both, the bioreactor and fermentor are industrially important in producing various products.
Working principle of solid fermenter:
Microorganisms grow on a moist, water-insoluble substrate for fermentation, and do not contain any free water during solid fermentation. With the increase of free water produced by microorganisms, the scope of solid fermentation extends to viscous fermentation and solid particle suspension fermentation. It can be considered that the growth environment is not necessarily the same between each cell. In order to improve the cultivation efficiency, the method of increasing the surface area is adopted. Labs generally use test tube slopes, petri dishes, triangular flasks, Kirschner flasks, etc. for cultivation. Most factories use curved plates, curtains, and ventilated curved ponds. Due to the selection of agricultural and sideline products such as bran as raw materials, the price is low, the particle surface area is large, loose and ventilated, and the raw materials are easy to obtain in large quantities. Therefore, it is widely used in the brewing industry. However, since there are still many difficulties in large-scale surface culture technology, in fermentation production, liquid surface culture can be used, and liquid submerged culture method is mostly used instead.
Structural characteristics of solid fermenter:
The solid fermenter can ensure the temperature required for fermentation, and the constant temperature control is carried out through the jacket. It can be sterilized in the tank or can be sterilized before entering the fermenter for fermentation. Humidity adjustment is generally carried out by a humidity controller to monitor the humidity in the tank. It uses a sterile water spray (fog) device and a water evaporation device so that the 0-100% humidity sensor device can be adjusted arbitrarily within its humidity range, maintain constant humidity and display. Ventilation control is also a major control point for solid fermenters. The requirement for ventilation is that it must be sterile, and the air volume and speed can be adjusted.
The setting of the stirring system is one of the most critical control points of the solid fermenter. Since solid fermentation inoculation requires uniformity and is not easy to mix, the design of stirring must ensure uniform mixing of materials and uniform distribution of bacteria after inoculation. Inoculation is generally carried out by spraying. Stirring forms include mechanical stirring and synchronous tank and mechanical stirring. Stirring adopts upper direct-coupled mechanical or horizontal shaft stirring, mechanical seal for kettle, various forms of high-performance stirring paddles, defoaming paddles, AC speed-regulating motor, and the speed is infinitely adjustable. Glass window, easy to observe the state of the tank. Various process interfaces, including: temperature and humidity interface, sterile sampling port, special large discharge port, liquid strain inoculation port, feeding port, pressure gauge port, air inlet, exhaust port, jacket upper and lower ports, etc.
1. Tank temperature control
The temperature of the tank will affect the rate of the enzyme reaction and the solubility of oxygen in the culture medium during the fermentation process, which is closely related to the growth of the bacteria, the synthesis of antibiotics and the dissolved oxygen.
Tank temperature control Tank temperature control mainly includes the control of heating and cooling. Among them, the heating amount is calculated by the process; the cooling amount refers to the cooling amount required after heating or sterilization and under the specified cooling time. The tank temperature control device consists of a heater and a cooling water solenoid valve. When the temperature in the fermentation tank is lower (or higher) than a certain set value, the system will automatically turn on the heater (or cooling water solenoid valve) to achieve The purpose of controlling the temperature of the fermenter.
The cooling amount is obtained by calculating the cooling amount required under the specified cooling time, and the heating amount is obtained by calculating the heat required under the specified heating time. From the enthalpy calculation and the heat transfer rate, the heat transfer area is obtained, that is, the area of the jacket or coil of the fermenter.
Only by calculating the length of the pipeline, the pressure of the medium and the pressure loss can the diameter of the heat exchange tube be determined, and then the diameter of the control valve. The diameter of the control valve is based on the control fluctuation of the culture temperature as the main parameter. If the control fluctuation of the culture temperature is used as a parameter to calculate, the opening degree of 60% to 80% can often be set as a stable value; if the sterilization heating time is to be taken into account, the value can be lower, and the culture temperature is controlled to fluctuate. The amount will be larger.
In a temperature control system with a hot and cold jacket, if the above-mentioned process parameters are calculated properly, the parameter control is relatively accurate, and the process parameters can be obtained by the calculation of the soaring curve or the empirical method. In the tank temperature control that takes into account both cultivation and sterilization, PID automatic control is used, and the fluctuation control range of the cultivation temperature is ±0.1 °C.
Among them, the differential action can improve the dynamic response speed, and the integral action can effectively eliminate the static error, so that the ambient temperature does not affect the tank temperature control. Of course, the design of the heat exchange jacket should be as thin as possible to reduce the fluctuations caused by the temperature control lag.
Sterilization control According to the process and GMP requirements, the fermentation equipment should be cleaned, disinfected or sterilized. The sterilization method commonly used in fermenters is moist heat sterilization. In the sterilization temperature control, the steam trap valve is often used in the pipeline of the advanced fermentation tank to replace the traditional steam trap, but there are certain requirements for the design of the sterilization system, the steam pipeline and the valve diameter.
In the design of the sterilization program, the steam passing time and the draining time interval should be matched. At the moment when the draining diaphragm valve is opened, the pressure of the tank body is almost unchanged, and the condensed water is effectively drained, and no cold spots appear in this process. In the sterilization temperature control, the temperature fluctuation period in the heating curve should be considered, and it is generally set to be officially timed 1 min after reaching the set temperature to meet the F0 value requirements of moist heat sterilization.
2. pH value control
The pH value of the fermentation broth is a comprehensive reflection of the acidity and alkalinity of various biochemical reactions during the fermentation process.
There are two types of pH value control and pH value feeding. The pH value control and feeding system is mainly composed of a peristaltic pump (or other conveying system) and a weighing system. In addition to the weighing system, these control systems are greatly affected by the pressure difference between the tanks, including pressure changes caused by the liquid level. The weighing system is also affected by the fluctuation of the liquid level, so it depends on the size of the tank and the ratio of diameter to height. Take into account the usage situation.
At present, the pH control device consists of an acid addition peristaltic pump and an alkali addition peristaltic pump. When the pH value is higher than the set value, the system automatically starts the acid addition peristaltic pump, and feeds acid into the fermenter in a time-proportional manner until the set value; when the pH value is lower than the set value, the alkali addition peristaltic pump starts to adjust.
Processing | Fermenting Equipment |
Applicable Industries | Manufacturing Plant, Food & Beverage Factory, Vaccine, Bacterial Culture,Pharmaceutical Industry |
Brand Name | FUL |
Processing Types | lactic acid bacteria,yeast,enzymes,microorganisms,Fungus,cell,bacterial |
Certification | ISO DIN ASME |
Warranty | 1 Year |
service | Field installation, commissioning and training |
Machinery Capacity | 50L, 100L, 200L, 300L, 400L, 500L, 600L, 700L, 800L, 1000L, 1200L, 1500L, 1800L, 2000L, 2500L, 3000L, 3500L, 4000L, 5000L, 10000L, 20000L, |
Machinery Test Report | Provided |
Core Components | Gearbox, Motor, Gear, Pump, Pressure vessel, PLC, OTHER, Bearing, Engine |
Key Selling Points | Automatic |
Video outgoing-inspection | Provided |
After-sales Service Provided | Engineers available to service machinery overseas |
Application | Culture bacterial yeast cell |
Control system | PLC With Touch Screen |
Function | PH/DO/Foam/Temp control |
Volume | 1L-200000L |
Material | Stainless steel 304/316 |
Manufacturer of Bioreactors and Fermenters
Manufacturer of Bioreactors and Fermenters
We are a dynamic company that specializes in engineering, developing, and manufacturing innovative bioprocess equipment. We manufacture a wide range of bioreactors, fermenters, at-line analyzers, CIP/SIP systems and process vessels for research, development, and production, suitable for fermentation, cell culture, stem cells, renewable technologies, algae, biomass, biofuel, hydrolysis and more.